The RNAstorm™ Fresh Cell and Tissue Kit provides high quality total RNA from either cultured cells or fresh tissue in as little as 20 minutes. High yields (up to 120 μg) can be obtained using a simple and quick column-based protocol. Contaminating DNA is removed using a DNase treatment step, and the protocol avoids toxic chemicals such as phenol or chloroform.
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Total RNA was extracted from 107 Jurkat cells, mouse liver tissue (~8 mg) and calf liver tissue (~17 mg) using the RNAstorm™ Fresh Cell and Tissue Kit and a leading competitor kit. Concentrations were measured using Qubit. For all samples, higher yields were obtained using the RNAstorm™ kit.
Electropherograms of total RNA from 106 Jurkat cells, extracted using the RNAstorm™ Fresh Cell and Tissue Kit and a leading competitor kit. Post extraction samples were analyzed for quality using the Agilent Bioanalyzer RNA 6000 Nano assay. The RNAstorm™ Fresh Cell and Tissue Kit delivered high quality total RNA with a RNA Integrity Number (RIN) of 10.
| Applications | NGS (RNA-Seq), gene expresssion array, PCR, qPCR |
| Kit format | Manual (50 spin columns included) |
| DNase treatment step | Included |
| Input sample |
Up to 107animal cells Up to 30 mg animal tissue |
| Isolation time | 20 minutes |
| Each kit includes: | Lysis Buffer Buffer FRB DNase I DNase Buffer Wash Buffer Spin Columns |
